自制溃疡液治疗口腔溃疡的疗效观察

目的观察自制溃疡液局部涂擦治疗口腔溃疡的疗效。方法将39例患口腔溃疡的患者随机分为治疗组19例,应用自制溃疡液局部涂擦每日4次,对照组20例采用多贝尔液含漱每日4次,观察口腔溃疡愈合时间。结果治疗组在口腔溃疡的愈合时间明显短于对照组,2组治愈时间经统计学处理,差异显著(P<0.01)。结论口腔溃疡液疗效确切,无明显不良反应,值得推广。     

3189例次深静脉导管的病原学检测结果分析

目的 探讨深静脉导管送检标本的病原学特征及细菌耐药性。方法 回顾分析2001-08～2006-05期间送检的3189例次深静脉导管标本的病原学结果及细菌耐药性。结果 3189份标本中，病原学阳性471份（14．77％）．共检出病原菌514株，G^＋菌、G^-菌和真菌分别占42．41％、40．66％和16．93％。五种最常见的病原菌分别为表皮葡萄球菌（15．56％）、金黄色葡萄球菌（13．81％）、铜绿假单胞菌（13．23％）、肺炎克雷伯菌（7．59％）和鲍曼不动杆菌（6．23％）：金黄色葡萄球菌中MRSA占91．18％，凝固酶阴性葡萄球菌中MRScn占88．57％，肠杆菌科中产ESBLs占12％；铜绿假单胞菌和鲍曼不动杆菌的耐药率呈上升趋势，并出现了全耐药菌株。结论 深静脉导管标本检出的病原菌以耐药菌为主，加强病原学监测以及导管护理．避免交叉感染．是控制深静脉导管相关感染发生的关键．     

丁香茄挥发油成分GC-MS分析

目的分析丁香茄中挥发油的化学成分。方法采用水蒸气蒸馏法提取丁香茄中挥发油,用GC-MS法测定和分析其化学组分。结果共鉴定了19个成分,占总峰面积的96%,按相对峰面积计,其中含亚油酸为62.8%,总脂肪酸和其他酯约占72%。结论丁香茄挥发油的主要成分为脂肪酸,为开发利用丁香茄资源提供了实验依据。     

运动锻炼对雌激素缺乏所致小鼠抑郁样行为的作用及炎症机制

目的 阐明运动锻炼对雌激素缺乏所致小鼠抑郁样行为的影响及其炎症机制,并为运动锻炼治疗更年期抑郁症提供理论依据.方法 将60只雌性小鼠随机分为假手术组(Sham组)、假手术+运动组(Sham+ Ex组)、去卵巢组(Ovx组)、去卵巢+运动组(Ovx+Ex组).建立假手术和去卵巢模型,术后1周,运动组进行为期5周的跑台锻炼.运用糖水实验、旷场实验和悬尾实验来观察各组小鼠的行为学改变情况.提取各组小鼠海马组织,用ELISA试剂盒检测5羟色胺(5-hydroxytryptamine,5-HT)、吲哚胺2,3-双加氧酶(indoleamine 2,3-dioxygenase,IDO)及炎性细胞因子的水平.结果 与Sham组比较,Ovx组小鼠发生了抑郁样行为改变,包括兴趣缺失、探究性行为减少、绝望情绪增加;运动锻炼可以改善Ovx小鼠抑郁样行为.与Sham组比较,Ovx组小鼠海马中5-HT含量降低(P＜0.0l),IDO含量升高(P＜0.05);给予Ovx小鼠运动锻炼能够逆转上述改变.与Sham组相比,Ovx组小鼠海马中促炎细胞因子IL-1β(P＜0.05)、IL-6 (P＜0.01)和IFN-γ(P＜o.01)含量增加,抑炎细胞因子IL-4 (P＜0.01)、IL-10 (P＜0.05)含量降低;给予Ovx小鼠运动锻炼能够逆转IL-lβ、IFN-γ和IL-4表达的改变.结论 运动锻炼可能通过减轻海马炎症反应来改善雌激素缺乏所致抑郁样行为.     

22种肿瘤标志物在大肠癌诊断的临床应用价值

ObjectiveAssessment of diagnostic value of multiple molecular tumor markers in detection of colorectal cancer in peripheral blood. MethodsA 1∶1 matched case control study was carried out to detect multiple molecular tumor markers of 20 cases of colorectal can cer patients and healthy controls in peripheral blood by using methods of Bio-Plex200 liquid chip. ResultsThe best cutoff value, sensitivity, specificity, kappa value of macrophage migration inhibitory factor (MIF) were 366.09, 95.0, 96.88 and 0.918 8 respectively; of vascular endothelial growth factor (VEGF) were 146.37, 100.0, 94.12 and 0.917 2 respectively; of fibroblast growth factor-2 (FGF-2) were 62.68, 90.0, 93.75 and 0.837 5 respectively. ConclusionMacrophage MIF, VEGF and FGF-2 has the best authenticity, reliability and predictability.     

Using aripiprazole to reduce antipsychotic-induced hyperprolactinemia: meta-analysis of currently available randomized controlled trials

Background

Hyperprolactinemia (HPL) is a common side effect of antipsychotic medications. Recent reports suggest that aripiprazole can ameliorate antipsychotic-induced HPL, but results are inconsistent and the single available systematic review only considered five studies.

Aim

Conduct an updated meta-analysis of all randomized controlled trials (RCTs) about the efficacy and safety of aripiprazole as an adjunctive treatment for antipsychotic-induced hyperprolactinemia.

Methods

English and Chinese databases were searched for RCTs about the use of aripiprazole in treating antipsychotic-induced HPL published by January 20, 2015. Studies were selected using pre-defined inclusion and exclusion criteria. The Cochrane Risk of Bias tool was used to evaluate risk of biases, the Cochrane GRADE measure was used to assess the quality of evidence, and Review Manager 5.3 software was used for data analysis.

Results

A total of 21 studies, 19 of which were conducted in mainland China, were included in the analysis. Meta-analysis of data from 8 of the studies with a pooled sample of 604 individuals found that compared to the control condition adjunctive aripiprazole significantly increased the proportion of participants who experienced HPL recovery (risk ratio [RR]=19.2, 95%CI=11.0-33.5). The proportion who experienced any adverse effect during follow-up did not differ between the two groups, but the aripiprazole group was more likely to report somnolence (RR=2.76, 95%CI=1.34-5.69) and headaches (RR=2.31, 95%CI=1.08-4.92). High-dose aripiprazole (>5mg/day) was more effective than low-dose (<5mg/day) aripiprazole (RR=30.0, 95%CI=10.2-120.7 v. RR=15.1, 95%CI=8.1-28.1), but this difference was not statistically significant. The risk of bias in the studies was rated as ‘high’ in 6 of the studies and ‘unclear’ in 15 studies, and the quality of evidence was rated as ‘high’ for only 7 of the 57 outcome measures assessed.

Conclusions

This study systematically reviewed and evaluated all relevant RCTs and found that adjunctive aripiprazole is effective and safe to use in the treatment of antipsychotic-induced HPL. However, the low quality of some of the studies, the incomplete methodological information provided for most of the studies, and the relatively short follow-up time of the studies raises question about the validity of the results. Further work that resolves these methodological and reporting issues is needed.     

Effect of somatostatin on postoperative gastrointestinal function and stress level in children with acute abdomen: a prospective randomized controlled study北大核心CSCD

目的 探讨生长抑素对急腹症患儿术后胃肠功能及应激水平的影响。 方法 选取2019年8月至2021年6月徐州市儿童医院收治的行手术治疗的102例急腹症患儿为研究对象。将患儿随机分为观察组和对照组,每组各51例。对照组患儿术后给予止血、抗感染等常规治疗,观察组在常规治疗的基础上加用生长抑素。术前、术后第1天及术后第5天采集两组患儿外周血,比较两组患儿血清血管内皮素-1（endothelin-1,ET-1）、促肾上腺皮质激素（adrenocorticotropic hormone,ACTH）、皮质醇（cortisol,Cor）及胃泌素、胃动素水平,以及两组患儿术后恢复情况及并发症发生率。 结果 术前两组患儿血清ET-1、ACTH、Cor、胃动素及胃泌素水平差异无统计学意义（P>0.05）。术后第1天、第5天,观察组患儿血清ET-1、ACTH、Cor水平均显著低于对照组（P<0.05）;术后第5天,观察组患儿胃动素与胃泌素水平均高于对照组（P<0.05）。术后观察组患儿首次肛门排气时间、肠鸣音恢复时间、首次排便时间、住院时间均较对照组缩短（P<0.05）。观察组并发症发生率（6%）显著低于对照组（24%,P<0.05）。 结论 生长抑素可显著降低急腹症患儿术后应激反应,改善胃肠功能,降低并发症发生率,有益于疾病预后。     

动态心电图诊断冠心病的价值分析

目的：比较12导联动态心电图与冠状动脉造影对冠心病的诊断价值。方法选取2011年11月~2014年9月本院拟诊为冠心病的114例患者作为研究对象,给予24 h动态心电图检查,以冠状动脉造影为标准进行对比分析。结果114例患者中,冠状动脉造影检查阳性84例,阴性30例,阳性率73.7%;其中单支病变36例,双支病变26例,多支病变22例。动态心电图诊断阳性66例,阴性48例,阳性率为57.9%,敏感性为70.2%,特异性为76.7%,假阳性率为23.3%,假阴性率为29.8%。随着冠状动脉病变支数的增多,动态心电图诊断冠心病的阳性率亦增高,差异有统计学意义（P<0.05）。结论动态心电图是目前开展的无创、安全、价廉的诊断冠心病方法之一,尤其对冠状动脉双支及多支病变的阳性检出率高,可作为冠心病诊断的初筛检查。     

屏气磁共振电影成像对冠心病患者左心室整体功能的评价

目的探讨屏气磁共振电影成像（Cine-MRI）对冠心病患者左心室整体功能的评价。方法对34例获得冠状动脉导管造影（CCA）结果的冠心病患者行Cine-MRI获得短轴位和垂直长轴位电影图像,测量左心室整体功能参数,以CCA判断的冠状动脉狭窄程度为金标准,对各项整体功能参数行统计学评价。结果冠心病患者表现为心肌质量和心室容积增加,前者具有相对较高的灵敏度、特异度、准确度,舒张期的改变较收缩期敏感;EF和SV下降,具有较高的特异度。结论屏气Cine-MRI能够较好地评价左心室整体功能,客观反映冠心病患者左心室整体功能的收缩、舒张状态。     

九味肝泰胶囊对高脂饮食诱导大鼠非酒精性脂肪肝的治疗作用

目的研究九味肝泰胶囊对高脂饮食诱导的大鼠非酒精性脂肪性肝病(NAFLD)的治疗作用和可能机制。方法采用高脂饮食诱导大鼠NAFLD动物模型。设对照组,模型组,水飞蓟素阳性对照组(0.05 g/kg),九味肝泰胶囊高、中、低剂量(1.80、0.90、0.45g/kg)组。除对照组给予普通饲料饲养外,其他各组以高脂饲料喂养,饲养10周。从第5周开始,4个给药组分别ig给予相应药物,共给药6周。观察和检测大鼠体质量、肝湿质量、肝脏指数和肝脏形态学变化;检测血清中的高密度脂蛋白-胆固醇(HDL-C)、游离脂肪酸(FFA)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)水平;检测肝脏组织中总胆固醇(TC)、三酰甘油(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果与模型组比较,九味肝泰胶囊高、中剂量组能显著降低NAFLD大鼠血清中的FFA、AST、ALT水平(P0.05),升高HDL-C水平(P0.05);显著降低NAFLD大鼠肝脏组织中的TC、TG、MDA水平(P0.05),升高SOD的活性(P0.05);NAFLD大鼠的肝组织病理学形态均得到不同程度改善,大鼠肝组织脂肪变程度减轻。结论九味肝泰胶囊对NAFLD大鼠具有调节脂质和保护肝脏的作用,其作用机制可能与减少脂质在肝脏的沉积、减轻过氧化损伤有关。